What size is a T75 flask?

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What Size is a T75 Flask?

T75 flasks are commonly used in scientific and research laboratories for cell culture applications. These flasks have a specific size that is designed to accommodate a certain amount of cell suspension and provide optimal conditions for cell growth and proliferation. In this article, we will explore the size of a T75 flask and its importance in the field of cell culture.

T75 flasks are a type of cell culture flask that is widely used in the scientific community. The "T" in T75 stands for "tissue culture," and the number 75 refers to the flask's working volume capacity, which is approximately 75 milliliters. These flasks are commonly used for the propagation and expansion of adherent mammalian cell lines.

Size and Dimensions of T75 Flasks.

T75 flasks typically have a flat bottom and a vented or non-vented cap. The exact size and dimensions of these flasks may vary slightly among different manufacturers, but they generally have a standard size. A typical T75 flask has a height of 115-120 millimeters and a diameter of 85-90 millimeters. The flask's neck has a diameter of around 30 millimeters, which allows for easy pouring and access for pipetting.

Working Volume and Media Volume in T75 Flasks.

As mentioned earlier, the working volume of a T75 flask is approximately 75 milliliters. However, it is important to note that the working volume does not represent the total volume that should be added to the flask. Typically, the recommended media volume for a T75 flask is around one-third to one-half of the working volume, which means you would add approximately 25-37.5 milliliters of media to the flask. This volume allows for proper gas exchange and prevents media evaporation during long incubation periods.

Usages of T75 Flasks.

T75 flasks are mainly used for cell culture experiments in research laboratories. These flasks provide a large surface area for cells to grow and attach to the flask's surface. The flat bottom design and moderate volume make them suitable for the expansion and maintenance of adherent cell lines. T75 flasks are typically used for routine cell culture, including passaging cells, expansion, and harvesting cells for downstream applications. They are also used for preparing cells for various assays, drug screening, and toxicity testing.

Conclusion.

T75 flasks have become an essential tool in cell culture laboratories due to their practical size and functionality. Their standard dimensions and working volumes make them suitable for routine cell culture experiments, and their design allows for optimal cell growth and attachment. Understanding the size and specifications of T75 flasks is crucial for researchers and scientists working in the field of cell culture.

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